urealyticus isolates of animal origin were shown to carry multiple antimicrobial resistance (AMR) genes and express phenotypic resistance to various classes of antimicrobials 9, 10, 11. cohnii isolates were recently detected on frequently touched surfaces in a London hospital 8. cohnii has been reported to be an opportunistic pathogen 6, 7. urealyticus should be regarded as an individual species S. However, recent phylogenetic studies suggested that the subspecies S. cohnii has been divided into the subspecies S. Among others, Staphylococcus ( S.) cohnii was found on farms however, it has been regarded a commensal bacterium, and not involved in severe animal infections such as bovine mastitis 2, 4. Non- aureus staphylococci (NAS) are frequently found on dairy farms and occasionally cause intramammary infections 1, 2, 3. urealyticus from selected German dairy farms show multiple resistances to antimicrobial substances and may carry unknown antimicrobial resistance determinants. aureus showed identities of only 70–80% with regard to PBP1, PBP2 and PBP3. A comparison with penicillin-binding protein (PBP) amino acid sequences of S. The specificity of cefoxitin susceptibility testing for mecA or mecC gene prediction in S.
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Although 14/15 isolates lacked the blaZ, mecA and mecC genes, they showed reduced susceptibility to a number of beta-lactam antibiotics including cefoxitin (MIC 4–8 mg/L) and penicillin (MIC 0.25–0.5 mg/L). The isolates harbored various AMR genes ( aadD1, str, mecA, dfrC/K, tetK/L, ermC, lnuA, fexA, fusF, fosB6, qacG/H) and exhibited non-wildtype phenotypes (resistances) against chloramphenicol, clindamycin, erythromycin, fusidic acid, rifampicin, streptomycin, tetracycline, tiamulin and trimethoprim. urealyticus isolates from German dairy farms were analyzed by whole-genome sequencing and AMR testing. urealyticus are known, they may serve as an antimicrobial resistance (AMR) gene reservoir for harmful staphylococcal species. Two rarely investigated species are Staphylococcus ( S.) cohnii and S. Non- aureus staphylococci are commonly found on dairy farms.